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PEPTIDE STORAGE


PEPTIDE STORAGE                                                                                                      http;//.www.acinopeptide.com                        

All Acinopeptide peptides are supplied lyophilized and should be stored and handled according to the following guidelines.

Storage Guidelines for Lyophilized Peptides:
Lyophilized peptides should be refrigerated and stored away from bright light. For short-term storage ranging from one week to two months, lyophilized peptides may be stored at 4℃. For long-term storage, lyophilized peptide should be stored at -20℃ in deep freezer. Even though this condition keeps most peptides stable for several years, peptides' long term stability is at risk when peptides expose to moisture, therefore, the vial should be tightly capped at all times. This also requires careful experiment planning to minimize vial openings.

Peptide Solutions Storage Guideline:
Do not store excessive peptides in solution because the shelf life of peptides in solution is very limited, much shorter than lyophilized peptides. This is especially true for peptides whose sequences contain cysteine, methionine, tryptophan, asparagine, glutamine, or N-terminal glutamic acid. It is necessary to aliquot the amount of peptide you need for the day and re-lyophilize the remaining peptide solution. Another drawback is that peptides stored in solution are susceptible to bacterial degradation. If storage of peptide in solution is unavoidable, use sterile buffers at pH 5-6 and store aliquots at -20℃ and pass the peptide solution through a 0.2 ?M filter to rid the bacteria.

Preparation for Use:
When preparing peptides for use, please consult Acinopeptide's following guidelines:
Warm the peptide vial to room temperature before opening.
Weigh out the desired quantity of peptide quickly.
Reseal the vial tightly.
Store the remaining peptide in a freezer, preferably below -20℃, under dry conditions.

Avoid repeated freeze and thaw cycles for both lyophilized peptides and peptide solutions. If frequent and periodical sample takings from storage are unavoidable, Acinopeptide recommends making a series of aliquots in advance. Due to the fact that peptides whose sequences containing cysteine, methionine, or tryptophan are prone to air oxidation. It is recommended that air be purged from the peptide storage vial and replaced with inert gases such as argon or nitrogen for storage.