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PEPTIDE ANALYSIS http://www.acinopeptide.com
Over the years, Acinopeptide has gathered an extensive know-how not only in the synthesis of peptides, but also in the analysis of these compounds. The implementation of suitable analytical methods, an experienced, well-trained staff, and state-of-the-art equipment are important prerequisites for Acinopeptide to maintain the superior quality of its products and to meet the expectations of even the most demanding customer.
We constantly develop and validate new analytical methods, aiming at setting industrial standards in the analysis of peptides. Currently, our products are analyzed by the following methods on a routine basis:
HPLC analysis
RP-HPLC (reversed phase)
SEC-HPLC (size exclusion)
IE-HPLC (ion exchange)
method develpment and validation for Non-GMP and GMP projects.
Mass Spectrometric analysis
ESI-MS full scan
LC-MS
LC-MS-MS
Amino Acid Analysis
Proteins/peptides are hydrolyzed using HCl. The released amino acid residues are analyzed on a reversed phase HPLC after pre-column derivatization.
Water Content
Moisture Analysis by Karl Fisher titration
Peptide content
by elemental analysis
Peptide counter ion content
TFA, Acetic Acid content by HPLC
Residual So